产品货号:
GS2100
中文名称:
HpaI内切酶
英文名称:
HpaI
产品规格:
1kU
发货周期:
1~3天
产品价格:
询价
储存条件:冷冻(-20℃)
使用说明
High enzyme concentration results in star activity.
技术规格
相关搜索:HpaI内切酶,HpaI限制性内切酶
使用说明
High enzyme concentration results in star activity.
技术规格
Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl. |
Recognition sites | GTT↑AAC |
来源 | E.coli strain,that carries the cloned gene Hpa I from Haemophilus parainfluenzae |
Assayed on | Lambda DNA |
Optimal buffer | Y?(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.) |
Optimal temperature | 37℃ |
Storage conditions | 10 mM Tris-HCl(pH 7.5);50 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycerol.Store at -20℃. |
Ligations | After 5-fold overdigestion with enzyme about 60%of the DNA fragments can be ligated and recut. |
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 5 u.a.of enzyme for 16hoursat 37℃. |
Methlation effect | Methylation sensitivity not tested |
Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
Campatible Ends | BstHPI,KspAI |
相关搜索:HpaI内切酶,HpaI限制性内切酶