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产品目录

HpaI内切酶图片
产品货号:
GS2100
中文名称:
HpaI内切酶
英文名称:
HpaI
产品规格:
1kU
发货周期:
1~3天
产品价格:
询价
储存条件:冷冻(-20℃)

使用说明

High enzyme concentration results in star activity.

技术规格

Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sitesGTT↑AAC
来源E.coli strain,that carries the cloned gene Hpa I from Haemophilus parainfluenzae
Assayed onLambda DNA
Optimal bufferY?(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.)
Optimal temperature37℃
Storage conditions10 mM Tris-HCl(pH 7.5);50 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycerol.Store at -20℃.
LigationsAfter 5-fold overdigestion with enzyme about 60%of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1μgof Lambda DNA with 5 u.a.of enzyme for 16hoursat 37℃.
Methlation effectMethylation sensitivity not tested
Thermal inactivationenzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible EndsBstHPI,KspAI

相关搜索:HpaI内切酶HpaI限制性内切酶
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